Improved oligosaccharide synthesis by protein engineering of β-glucosidase CelB from hyperthermophilic Pyrococcus furiosus

✍ Scribed by Therese Hansson; Thijs Kaper; John van der Oost; Willem M. de Vos; Patrick Adlercreutz

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 320 KB
• Volume: 73
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.1052

✦ Synopsis

Abstract

Enzymatic transglycosylation of lactose into oligosaccharides was studied using wild‐type β‐glucosidase (CelB) and active site mutants thereof (M424K, F426Y, M424K/F426Y) and wild‐type β‐mannosidase (BmnA) of the hyperthermophilic Pyrococcus furiosus. The effects of the mutations on kinetics, enzyme activity, and substrate specificity were determined. The oligosaccharide synthesis was carried out in aqueous solution at 95°C at different lactose concentrations and pH values. The results showed enhanced synthetic properties of the CelB mutant enzymes. An exchange of one phenylalanine to tyrosine (F426Y) increased the oligosaccharide yield (45%) compared with the wild‐type CelB (40%). Incorporation of a positively charged group in the active site (M424K) increased the pH optimum of transglycosylation reaction of CelB. The double mutant, M424K/F426Y, showed much better transglycosylation properties at low (10–20%) lactose concentrations compared to the wild‐type. At a lactose concentration of 10%, the oligosaccharide yield for the mutant was 40% compared to 18% for the wild‐type. At optimal reaction conditions, a higher ratio of tetrasaccharides to trisaccharides was obtained with the double mutant (0.42, 10% lactose) compared to the wild‐type (0.19, 70% lactose). At a lactose concentration as low as 10%, only trisaccharides were synthesized by CelB wild‐type. The β‐mannosidase BmnA from P. furiosus showed both β‐glucosidase and β‐galactosidase activity and in the transglycosylation of lactose the maximal oligosaccharide yield of BmnA was 44%. The oligosaccharide yields obtained in this study are high compared to those reported with other transglycosylating β‐glycosidases in oligosaccharide synthesis from lactose. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 203–210, 2001.