✦ LIBER ✦

Improved molecular weight analysis of streptococcal hyaluronic acid by size exclusion chromatography

✍ Scribed by David C. Armstrong; Michael R. Johns

Publisher: Springer-Verlag
Year: 1995
Tongue: English
Weight: 444 KB
Volume: 9
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00159564

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✦ Synopsis

Four size exclusion chromatography (SEC) calibration techniques were tested for use in the molecular weight characterisation of Streptococcal Hyahrronic Acid (HA). An exponential equation, evaluated using the Hamielec method, was superior to the customary peak position method. It provided the most accurate estimates of the weight average molecular weight, Mw. The calibration was valid for HA in the range 800 -2500 kDa, and permitted the calculation of both polydispersity and molecular weight distributions for HA from Streptococcal fermentations. This exponential calibration approach should have application in the characterisation of other large biopolymers, particularly where pore size of available SEC media is limiting.

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