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Improved methods for ultracryotomy of CNS tissue for ultrastructural and immunogold analyses

โœ Scribed by Takumi Akagi; Kinji Ishida; Tomohito Hanasaka; Shuichiro Hayashi; Masahiko Watanabe; Tsutomu Hashikawa; Koujiro Tohyama

Book ID
108186306
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
618 KB
Volume
153
Category
Article
ISSN
0165-0270

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An enzymatic method for measuring picomo
An enzymatic method for measuring picomole quantities of acetylcholine and choline in CNS tissue
โœ P.A. Shea; M.H. Aprison ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 823 KB

A rapid and sensitive enzymatic assay for measuring picomole quantities of both acetylcholine (ACh) and choline (Ch) in tissue extracts has been developed. After ACh and Ch were extracted into 15% 1 N formic acid/85% acetone by the procedure of Toru and Aprison, lipids were removed by a heptane-chlo