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Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue

✍ Scribed by Shimada, Akemi; Komatsu, Koichiro; Nakashima, Kazuhisa; Pöschl, Ernst; Nifuji, Akira

Book ID
113040997
Publisher
Springer
Year
2012
Tongue
English
Weight
484 KB
Volume
137
Category
Article
ISSN
1432-119X

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The b-galactosidase activity encoded by the lacZ gene of Escherichia coli is widely used to monitor successful expression of transfected genes. Fluorogenic substrates allow detection of enzyme activity in viable cells, which, subsequently, can be selected for further study on the basis of fluorescen