Improved methodologies for the isolation and purification of tropoelastin

✍ Scribed by Judith Ann Foster; Celeste B. Rich; Marcia D. DeSa; Andrew S. Jackson; Sharon Fletcher

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 406 KB
• Volume: 108
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90575-8

✦ Synopsis

Increased yields of purified tropoelastin were obtained from lathyritic chicks which had been raised on normal commercial starting feed for 1 week followed by 8 days on commercial starting feed supplemented with 0.1% aminoacetonitrile .HCl and 0.05% e-aminocaproic acid. The increased age of chicks prior to lathyrogen administration plus the addition of the trypsin-like enzyme inhibitor, .z-aminocaproic acid, to the diet resulted in a 110% increase in the yield of tropoelastin. In addition, a cation-exchange system was developed for the fractionation of tropoelastin and removal of minor acidic protein contaminants.