✦ LIBER ✦
Improved method for the transformation ofArabidopsis thalianawith chimeric dihydrofolate reductase constructs which confer methotrexate resistance
✍ Scribed by Elke Kemper; Christoph Grevelding; Jeff Schell; Robert Masterson
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 465 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
✦ Synopsis
A modified root explant transformation method has been developed that is effective in producing transgenic Arabidopsis thaliana plants which are methotrexate resistant due to the integration of T-DNA vectors containing a chimeric dihydrofolate reductase gene. Molecular analysis shows that transformed methotrexate resistant plants contain the expected T-DNA construct with the chimeric gene. This transformation method also works well with other plant selectable markers, including hygromycin phosphotransferase and neomycin phosphotransferase II.