Improved method for measuring C1rC1s-(c1 inh)2 complexes by an enzyme-linked immunosorbent assay
✍ Scribed by Kenneth P. Mathews; Jack H. Herschbach; Sandra L. Chambers; Bruce L. Zuraw
- Book ID
- 102878741
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 961 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
Measurement of ClT-ClS-(Cl inh)2 complexes in serum or plasma by enzyme-linked immunosorbent assay (ELISA) has been proposed as a relatively convenient and sensitive means for assessing C1 activation. However, interference by unactivated C1 q (1-54~ at low serum or plasma dilutions has resulted in estimates that vary widely with the degree of serum or plasma dilution. Precipitating the interfering C1 q (r-+ with 6% polyethylene glycol has been proposed to resolve this problem, but here it is shown that this procedure also precipitates or coprecipitates some of the ClT-ClB(C1 inh)2 complexes. Satisfactory results have been achieved without PEG precipitation by testing high plasma dilutions under conditions where there is a sufficient excess of anti-C1 s coating the microtitration plate wells that removal of C1 q (r-s)* is not necessary. Optimizing conditions for quantitating these complexes at high dilution have been investigated. The mean normal EDTA plasma ClT-ClS-(Cl inh)p complex measurement was 36.6k7.0 (S.D.) ELISA units with a 95% confidence interval of 19.5-47.6~.
Besides providing a sensitive assay for C1 activation, measuring ClT-Cl %(C1 inh)2 complexes may help to clarify the pathophysiologic mechanisms resulting from C1 inh deficiency under various conditions.