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Improved manual sequential analysis of peptides

✍ Scribed by Mark Boehnert; David H. Schlesinger


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
393 KB
Volume
96
Category
Article
ISSN
0003-2697

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Autoradiography of 3H-labeled dansyl amino acids was applied to the sequential analysis of peptides available only in subnanomolar quantities. The method employs time-course analysis of the digestion of the peptide by aminopeptidase M, carboxypeptidase B, and dipeptidylaminopeptidase I. The cleaved