Improved manual sequential analysis of peptides
β Scribed by Mark Boehnert; David H. Schlesinger
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 393 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0003-2697
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A novel apparatus for performing manual gas-phase Edman chemistry on protein and peptide samples is described. Edman chemistry is performed in 6 to 10 Teflon continuous flow reactors (CFR), previously described by J. E. Shively et al. (1987) Anal. Biochem. 163, 5 17-529). The CFRs are packed with IO
Autoradiography of 3H-labeled dansyl amino acids was applied to the sequential analysis of peptides available only in subnanomolar quantities. The method employs time-course analysis of the digestion of the peptide by aminopeptidase M, carboxypeptidase B, and dipeptidylaminopeptidase I. The cleaved