Improved kinetic analysis of cytosolic free calcium in pressure-sensitive neuronal cells by fixed-time flow cytometry
✍ Scribed by Attila Tárnok
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 610 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0196-4763
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✦ Synopsis
Two flow cytometric techniques were used to measure rapid transient changes in [Ca'+], in the neuronal cell line NH15-CA2. Using on-line injection, the cell suspension and stimulating solution are mixed and delivered to the detection point by a rapid increase in sample pressure. In NH15-CA2, injection of medium alone resulted in [Ca2+Ii increase. Using the fixed-time method, where cells are maintained at constant pressure, no [Ca2+Ii increase was observed with medium alone. These results show that a rapid pressure increase alone alters the [Ca'+], in NH15-CA2 cells. Both methods showed similar kinetics of [Ca'+], in response to bradykinin but the fixed-time method was found to be better for determination of the percentage of responsive Cells.