Improved high-speed counter-current chromatography with three multilayer coils connected in series : II. Separation of various biological samples with a semi-preparative column

✍ Scribed by Yoichiro Ito; Hisao Oka; Wei Lee Yue

• Book ID: 104145645
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 657 KB
• Volume: 498
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84245-x

✦ Synopsis

The semipreparative capability of the newly developed high-speed counter-current chromatograph equipped with a set of three multilayer coils has been demonstrated in separations of a variety of biological samples including triterpenoic acids, indole auxins, bacitracin, flavonoids and tetracycline derivatives, each with a suitable two-phase solvent system. The sample quantities ranging from 50 to 500 mg were efficiently separated within a few hours. The separation of tetracycline derivatives was remarkably improved by adding ammonium acetate to the solvent system.