Improved high-performance liquid chromatography of sphingomyelin

✍ Scribed by Jon I. Teng; Leland L. Smith

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 435 KB
• Volume: 322
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97679-4

✦ Synopsis

The analysis of spingomyelin preparations from various sources has been recently accomplished by reversed-phase high-performance liquid chromatography (HPLC)', argentation chromatography* and mass spectrometry (MS) directly314 or in association with chromatography and derivatization5-s. By contrast, routine preparation of individual sphingomyelin molecular species for other purposes is not so well advanced. Moreover, in our hands prior chromatographic methods did not provide pure underivatized individual species required for planned peroxidation studies. Accordingly, we sought an improved HPLC system providing the required resolution. We describe herein such a reversed-phase HPLC system using linear gradient elution and binary and ternary solvent mixtures, with component analysis by chemical ionization mass spectrometry (CIMS).