Improved high-performance liquid chromatographic separation of the Stevia rebaudiana sweet diterpene glycosides using linear gradient elution
✍ Scribed by H.C. Makapugay; N.P.D. Nanayakkara; A.D. Kinghorn
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 443 KB
- Volume
- 283
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Products obtained from the Paraguayan herb Stevia rebaudiana (Bertoni) Bertoni are currently commercially available for sweetening purposes in both Japan and Brazi11~2. In Paraguay, teas made from S. rebaudiana are prescribed by physicians for the treatment of diabetes, as a result of the reported hypoglycemic activity of this plant3. The cultivation of S. rebaudiana has been reported to occur not only in Japan, Brazil and Paraguay, but also in South Korea, Taiwan, Thailand, Indonesia and Laos1.3-s. Climatic conditions in California are also suitable for the growth of this specie@. In addition, interest in S. rebaudiana has recently been expressed in the People's Republic of China'.
Several investigators have devised high-performance liquid chromatographic (HPLC) analytical methods for the separation and quantitation of the S. rebaudiana sweet steviol(13-hydroxy-ent-kaurenoic acid) glycosides+". Such methods have been applied either to plant materia18-10~12~1 s*l 6 or to products13,14,17 containing these sweet compounds. However, the separation of all eight of the known S. rebaudiana sweet diterpene glycos ides, namely stevioside, steviolbioside, rebaudiosides A-E and dulcoside A, has been achieved by only one group to datels*16. In the present communication, we report a more rapid, better resolved HPLC separation of the eight known S. rebaudiana sweet steviol glycosides, using an NH2 phase-bonded column with linear gradient elution. The applicability of this method to the analysis of S. rebaudiana leaf samples of diverse geographic origin is demonstrated.