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Improved high-performance liquid chromatographic analysis of intracellular deoxyribonucleoside triphosphate levels

✍ Scribed by Ronald A. Rimerman; Gregory D. Prorok; Kaey L. Cordel; Ann M. Shahwan; William P. Vaughan

Publisher
Elsevier Science
Year
1993
Weight
632 KB
Volume
619
Category
Article
ISSN
0378-4347

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Determination of ribonucleoside triphosp
Determination of ribonucleoside triphosphates and deoxyribonucleoside triphosphates in Novikoff hepatoma cells by high-performance liquid chromatography
✍ Franco Arezzo πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 556 KB

A rapid, specific, and sensitive method has been developed for the determination of ribonucleoside and deoxyribonucleoside triphosphates in Novikoff hepatoma cells. A simple three-step procedure was used. Extraction of the biological material with 5% cold trichloroacetic acid (TCA); elimination of T