Improved glycosylation of a foreign protein by Tn-5B1-4 cells engineered to express mammalian glycosyltransferases

Glycosylation of a recombinant protein i

Glycosylation of a recombinant protein in the Tn5B1-4 insect cell line: Influence of ammonia, time of harvest, temperature, and dissolved oxygen

✍ Michael Donaldson; H. Alan Wood; Peter C. Kulakosky; Michael L. Shuler 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 215 KB 👁 2 views

Glycosylation is both cell line and protein dependent. Culture conditions can also influence the profile of glycoforms produced. To examine this possibility in the insect cell/baculovirus system, structures of N-linked oligosaccharides attached to SEAP (human secreted alkaline phosphatase), expresse