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Improved expression of secreted and membrane-targeted proteins in insect cells

✍ Scribed by Richard B. Hitchman; Robert D. Possee; Evangelia Siaterli; Kevin S. Richards; Amber J. Clayton; Louise E. Bird; Raymond J. Owens; David C. J. Carpentier; Fiona L. King; John O. Danquah; Karen G. Spink; Linda A. King

Book ID
119980421
Publisher
Portland Press
Year
2010
Tongue
English
Weight
373 KB
Volume
56
Category
Article
ISSN
0885-4513

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High-level expression of secreted glycop
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An expression cassette for continuous highlevel expression of secreted glycoproteins by transformed lepidopteran insect cells has been developed as an alternative to baculovirus and mammalian cell expression systems. The expression cassette utilizes the promoter of the silkmoth cytoplasmic actin gen