Improved enzymatic hydrolysis of hair

✍ Scribed by C Offidani; S Strano Rossi; M Chiarotti

• Book ID: 103902372
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 227 KB
• Volume: 63
• Category: Article
• ISSN: 0379-0738
• DOI: 10.1016/0379-0738(93)90271-b

✦ Synopsis

An enzymatic hair extraction method is proposed for drug analysis. Pronase digestion of various aliquots of hair from a cocaine abuser was preceded by a 2-h incubation with a dithiothreitol solution. The extraction solution was tested to identify possible interferences in the radioimmunoassay and was compared with other hydrolysis methods to assess the results of extraction.