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Improved efficiency and stability of multiple cloned gene insertions at the δ sequences ofSaccharomyces cerevisiae

✍ Scribed by F. W. F. Lee; N. A. Da Silva

Publisher
Springer
Year
1997
Tongue
English
Weight
305 KB
Volume
48
Category
Article
ISSN
1432-0614

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The chromosomal 6 sequences of the yeast Saccharomyces cerevisiae were employed as recombination sites to integrate the bacterial neo' gene and the yeast SUC2 gene into the yeast genome. A dominate selection method employing the aminoglycoside antibiotic G418 was used. Transformation efficiencies an