Improved detection limits for electrospray ionization on a magnetic sector mass spectrometer by using an array detector
✍ Scribed by Robert B. Cody; Jun Tamura; Jeffrey W. Finch; Brian D. Musselman
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 749 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1044-0305
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✦ Synopsis
Array detection was compared with point detection for solutions of hen egg-white lysozyme, equine myoglobin, and ubiquitin analyzed by electrospray ionization with a magnetic sector mass spectrometer. The detection limits for samples analyzed by using the array detector system were at least 10 times lower than could be achieved by using a point detector on the same mass spectrometer. The minimum detectable quantity of protein corresponded to a signal-to-background ratio of approximately 2∶1 for a 500 amol/μL solution of hen egg-white lysozyme. However, the ultimate practical sample concentrations appeared to be in the 10-100 fmol/μL range for the analysis of dilute solutions of relatively pure proteins or simple mixtures.