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Importance of Phosphoenolpyruvate Carboxylase of Corynebacterium glutamicum during the Temperature Triggered Glutamic Acid Fermentation

✍ Scribed by S. Delaunay; D. Uy; M.F. Baucher; J.M. Engasser; A. Guyonvarch; J.L. Goergen

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 234 KB
Volume: 1
Category: Article
ISSN: 1096-7176
DOI: 10.1006/mben.1999.0131

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✦ Synopsis

To give clues about the respective importance of phosphoenolpyruvate carboxylase (PEPc) and pyruvate carboxylase (Pc) in Corynebacterium glutamicum metabolism during a temperature triggered glutamic acid fermentation, PEPc activity was genetically amplified and Pc activity was suppressed by biotin limitation in the culture medium. In absence of Pc activity, glutamate production was dramatically reduced whereas lactate excretion was strongly increased. Whereas PEPc amplification in excess of biotin (4 mg Â L) only slightly modified the cell kinetics, under biotin limiting conditions this amplification strongly improved the glutamate production (4 +g Â L). When Pc was absent, PEPc activity was sufficient to allow up to 700 of the maximal glutamate production rate and seemed to have an important anaplerotic role, especially at the beginning of the production phase. In contrast, Pc was predominant during the remainder of the glutamate fermentation.

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