𝔖 Bobbio Scriptorium
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Importance of light dose in fluorescent microscopy

✍ Scribed by Malcolm W.R. Reed; Frederick N. Miller

Book ID
116035710
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
235 KB
Volume
36
Category
Article
ISSN
0026-2862

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## Abstract In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi‐illumination modes. In wide‐field imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform il