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Implications for the cis-requirements for Ds transposition based on the sequence of the wxB4 Ds element

✍ Scribed by Varagona, Marguerite ;Wessler, Susan R.

Publisher
Springer
Year
1990
Tongue
English
Weight
609 KB
Volume
220
Category
Article
ISSN
0026-8925

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✦ Synopsis

The nucleotide sequence of the 1494 bp wxB4 Ds element is presented. A comparison with previously characterized Ds elements reveals several novel features. This element has less Ac terminal sequence than other Aclike Ds elements. The left terminus contains 398 bp of Ac sequence interrupted by a transposon-like D N A insertion, leaving only 317 bp of contiguous Ac sequence. The right terminus has 259 bp of Ae terminal sequence. The interior of the element contains sequences not found in other cloned members of the AciDs family. We suggest that the role of this non-Ac D N A is to separate the Ac termini by a minimum distance and may be a eis requirement for Ds transposition in maize.

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