Implication of PKC isozymes in the release of biogenic amines by mussel hemocytes: effect of PDGF, IL-2, and LPS

✍ Scribed by M. Gonzalez-Riopedre; R. Barcia; J.I. Ramos-Martínez

• Book ID: 102337429
• Publisher: Wiley (John Wiley & Sons)
• Year: 2009
• Tongue: English
• Weight: 243 KB
• Volume: 311A
• Category: Article
• ISSN: 1932-5223
• DOI: 10.1002/jez.566

✦ Synopsis

Abstract

The innate immune system of marine mussels (Mytilus galloprovincialis) is operated by phagocytic cells termed hemocytes. Lipopolysaccharide (LPS), interleukin‐2 (IL‐2), or platelet‐derived growth factor (PDGF) increase biogenic amine synthesis in these cells, and the enzymes Ca^2+^‐independent protein kinase C (PKC) (p105/108) and Ca^2+^‐dependent PKC (p60) are involved in these processes. Stimulation by PDGF induces a down‐regulation process affecting the form p108 of the Ca^2+^‐independent PKC. In addition, PDGF produces the increase of expression of p60 in the membrane fraction. IL‐2 induces the disappearance of p108 from the membrane but does not affect the presence of p60 in cytosol or membrane. For its part, LPS activates exclusively p60 by a down‐regulation mechanism. The ensemble of results suggests that each agonist starts a pathway that implicates the PKC isoenzymes that mediate the regulation of the activities dopa decarboxylase, dopamine β‐hydroxilase, and phenyletanolamine N‐methyltranferase, which lead to different actions related to biogenic amine synthesis. J. Exp. Zool. 311A: 727–734, 2009. © 2009 Wiley‐Liss, Inc.