𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Implantation of mouse histocompatibility antigens into membranes of cultured tumor cells

✍ Scribed by David Julian Volsky; Lars Ährlund-Richter; Tina Dalianis; George Klein

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
421 KB
Volume
11
Category
Article
ISSN
0014-2980

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✦ Synopsis

Abstract

Membranes of murine lymphoma cells expressing H‐2^a^ antigens were isolated, purified and co‐reconstituted with isolated Sendai virus envelopes according to a previously published procedure (Volsky, D. J. et al., Proc. Natl. Acad. Sci. USA 1979. 76: 5440.). The resulting hybrid H‐2^a^/Sendai virus envelope vesicles (SH‐2^a^ vesicles) were capable of binding to and fusing with mouse lymphoma cells. The fusion resulted in the implantation of H‐2^a^ antigens into membranes of target cells, as demonstrated by the presence of serologically active antigens on cultured cells 8 and 16 h after implantation.

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