Impairment of hippocampal long-term potentiation by Alzheimer amyloid β-peptides

✍ Scribed by Qi-Sheng Chen; Bruce L. Kagan; Yutaka Hirakura; Cui-Wei Xie

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 196 KB
• Volume: 60
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(20000401)60:1<65::aid-jnr7>3.0.co;2-q

✦ Synopsis

Although it is generally believed that amyloid ␤ (A␤) peptides contribute to the pathogenesis of Alzheimer's disease, the precise role of these peptides in the development of memory loss of Alzheimer's disease, has not been fully understood. The present study examined the effect of several synthetic A␤ peptides on long-term potentiation (LTP), a cellular model of learning and memory, in rat hippocampal slices. Brief perfusion of slices with low concentrations (200 nM or 1 M) of A␤ 1-42 , A␤ 1-40 or their active fragment A␤ 25--35 significantly inhibited LTP induction without affecting the basal synaptic transmission and posttetanic potentiation in the dentate medial perforant path. A similar effect of A␤ 25-35 was also observed in the Schaffer colleteral-CA1 pathway. When comparing actions of several A␤ variants derived from A␤ 25-35 , the N-terminal sequence of A␤ 25-35 was found necessary for inhibiting LTP. In addition, A␤ variants lacking neurotoxic action and aggregating property were also able to block LTP, suggesting that this effect was neurotoxicity independent. Our findings demonstrated that subneurotoxic concentrations of A␤ peptides could strongly suppress long-term synaptic plasticity in the hippocampus. Such an effect might underlie the memory deficits seen in Alzheimer's disease before neuronal cell loss.