Impaired retrograde axonal transport from a nerve crush in streptozotocin diabetic rats

✍ Scribed by P. Sidenius; J. Jakobsen

• Publisher: Springer
• Year: 1980
• Tongue: English
• Weight: 673 KB
• Volume: 19
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00275273

✦ Synopsis

The axonal transport of proteins in crushed nerves of streptozotocin (40 mg/kg) diabetic rats was investigated 4 weeks after induction of diabetes. 35S-methionine was used as a marker for protein and 3H-fucose as a marker for glycoprotein. The precursors were injected into the fifth lumbar spinal ganglion and the accumulation of TCA-insoluble activity proximal and distal to a sciatic nerve ligature was measured at different time intervals after application of a crush. The start of accumulation distal to the ligature was delayed by 1 hour for proteins as well as for glycoproteins. Furthermore, the total amount of accumulated protein after 19 h was decreased by 18% while the decrease was 21% for glycoprotein. By insulin treatment the differences could both be prevented and reversed after 3 days of normoglycaemia. These findings demonstrate an impaired response to a nerve crush and might be the explanation for the regenerative abnormalities of peripheral nerves in diabetes.