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Impaired echinocytic transformation of ankyrin- and spectrin-deficient erythrocytes in mice

✍ Scribed by Dr. Walter H. Reinhart; L.-P. Amy Sung; K.-L. Sung; Seldon E. Bernstein; Shu Chien

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 598 KB
Volume: 29
Category: Article
ISSN: 0361-8609
DOI: 10.1002/ajh.2830290404

No coin nor oath required. For personal study only.

✦ Synopsis

The membrane skeleton of the red blood cell plays an Important role In the determination of cell deformablllty and cell shape. Under various in vitro conditions, red blood cells undergo an echinocytic or stomatocytic shape transformation. The mechanism of this fundamental process Is not well understood. We have studied the red cell shape transformation In normoblastlc anemia mice (nbhb) and spherocytic anemia mice (sphhph), which are deficient in ankyrin and spectrln, respectively. We found that both ankyrlndeflclent cells (nbhb) and spectrln-deficient cells (sphlsph) have a reduced capacity to undergo echinocytic transformation with various echlnocytogenlc treatments, that Is, Incubation with sodlum sallcylate (40 and 120 mM), calcium loading (50 pM A23187 + 2.2 mM Ca2+), or metabolic depletion (24 hr at 37OC). These results suggest that the functional Integrity of the membrane skeleton Is essential for the maintenance and transformation of the red cell shape.

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