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Impact of various glycosaminoglycans upon cAMP-dependent protein kinase

✍ Scribed by Joachim Dittmann; Carsten Krischek; Günther Harisch

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
167 KB
Volume
17
Category
Article
ISSN
0263-6484

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✦ Synopsis

The physiological eects of the second messenger cAMP are displayed by cAMP-dependent protein kinase-medicated phosphorylation of speci®c target proteins which in turn control diverse cellular functions. We have determined this enzyme substrate phosphorylation in the presence of various glycosaminoglycans using a cAMP-dependent protein kinase isolated from rat liver. The results indicate that sulfated and unsulfated polysaccharides are able to inhibit phosphorylation of histone type IIa catalysed by cAMP-dependent protein kinase. Based on their impact upon substrate phosphorylation, glycosaminoglycans can be divided into three groups: group I with the highest inhibitory eect: dermatan sulfate and heparan sulfate; group II: chondroitin 4-sulfate and group III with the lowest inhibitory eect: chondroitin 6-sulfate, keratan sulfate and hyaluronic acid.

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