Impact of rRNA methylations on ribosome recycling and fidelity of initiation in Escherichia coli
β Scribed by Anuradha Seshadri; Badrinath Dubey; Michael H. W. Weber; Umesh Varshney
- Book ID
- 110836253
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 781 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0950-382X
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The effect of three different types of mutations in ribosomal protein S5 of Escherichia coli on translational fidelity has been studied. Two of them, namely that conferring resistance to spectinomycin and that selected for partial suppression of a temperature-sensitive analyl-tRNA synthetase mutatio
Strains carrying both the ramA1 and the neaA301 mutations do not exhibit the restriction of informational suppressors normally associated with resistance to neamine. Furthermore, ribosomes from such strains exhibit increased misreading in vitro with respect to particles from the neaA strain. These p