𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Impact of a probiotic Enterococcus faecalis in a gnotobiotic mouse model of experimental colitis

✍ Scribed by Micha Hoffmann; Anja Messlik; Sandra C. Kim; Ryan B. Sartor; Dirk Haller

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
353 KB
Volume
55
Category
Article
ISSN
1613-4125

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Scope: IL‐10‐deficient (IL‐10^−/−^) mice are susceptible to the development of chronic intestinal inflammation in response to the colonization with commensal Enterococcus faecalis isolates. The aim of this study was to characterize the impact of a probiotic E. faecalis strain in germ‐free, wild‐type (WT), and disease‐susceptible IL‐10^−/−^ mice.

Methods and results: The probiotic E. faecalis and the colitogenic control strain OG1RF induced IL‐6 and IFN‐γ inducible protein‐10 secretion in the murine intestinal epithelial cell line Mode K. Epithelial cell activation involved nuclear factor κ B, p38 and extracellular signal‐regulated kinase 1/2‐dependent pathways. Mouse embryonic fibroblasts from WT and toll‐like receptor‐2‐deficient (TLR‐2^−/−^) mice confirmed that both E. faecalis strains trigger pro‐inflammatory responses via the pattern recognition receptor TLR‐2. Monoassociation of germ‐free IL‐10^−/−^ mice with the probiotic E. faecalis strain revealed pro‐inflammatory epithelial cell activation and colonic tissue pathology. The non‐pathogenic nature of E. faecalis was confirmed in monoassociated WT mice. 2‐DE and MALDI‐TOF MS identified the ER stress chaperone Hspa5 (glucose‐regulated protein 78) and 3‐mercaptopyruvate sulfurtransferase as key targets in the epithelium from IL‐10^−/−^ and TLR‐2^−/−^ mice.

Conclusion: This study shows the potential of probiotic bacteria to initiate pro‐inflammatory responses in the disease‐susceptible but not the normal host.

📜 SIMILAR VOLUMES

Relationship between inflammation and ti
Relationship between inflammation and tissue hypoxia in a mouse model of chronic colitis
✍ Norman R. Harris; Patsy R. Carter; Amit Singh Yadav; Megan N. Watts; Songlin Zha 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 369 KB

Background: Hypoxia has been reported to be associated with the colonic inflammation observed in a chemically induced mouse model of self-limiting colitis, suggesting that low tissue oxygen tension may play a role in the pathophysiology of inflammatory tissue injury. However, no studies have been re

Prevention of colitis-associated carcino
Prevention of colitis-associated carcinogenesis in a mouse model by diet supplementation with ursodeoxycholic acid
✍ Christoph Loddenkemper; Sylvia Keller; Marie-Loise Hanski; Minh Cao; Gerhard Jah 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 French ⚖ 356 KB

## Abstract Bile acids in the intestinal lumen contribute to the homeostatic regulation of proliferation and death of the colonic epithelial cells: Deoxycholic acid (DCA) appears to enhance and ursodeoxycholic acid (UDCA) to attenuate the process of chemically induced carcinogenesis. We studied the

5-aminosalicylic acid inhibits colitis-a
5-aminosalicylic acid inhibits colitis-associated colorectal dysplasias in the mouse model of azoxymethane/dextran sulfate sodium-induced colitis
✍ Margie L. Clapper; Monique A. Gary; Renata A. Coudry; Samuel Litwin; Wen-Chi L. 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 304 KB 👁 1 views

## Background: The impact of the antiinflammatory agent 5-aminosalicylic acid (5-ASA) on the risk for colitis-associated colorectal cancer remains controversial. The chemopreventive activity of 5-ASA was evaluated in the Swiss Webster model of azoxymethane (AOM)/dextran sulfate sodium (DSS)-induce

The impact of human superoxide dismutase
The impact of human superoxide dismutase 1 expression in a mouse model on the embryotoxicity of hydroxyurea
✍ Geneviève Larouche; Barbara F. Hales 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 245 KB 👁 2 views

## Abstract ## BACKGROUND: Oxidative stress is hypothesized to mediate embryotoxicity during organogenesis, yet the reactive oxygen species involved are not defined. The superoxide oxygen radical is converted to hydrogen peroxide, a less reactive species, by superoxide dismutases (SODs). If supero

The impact of glycosylation on HLA–DR1–r
The impact of glycosylation on HLA–DR1–restricted T cell recognition of type II collagen in a mouse model
✍ Alexei von Delwig; Daniel M. Altmann; John D. Isaacs; Clifford V. Harding; Rikar 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB 👁 1 views

## Abstract ## Objective Type II collagen (CII) is a candidate autoantigen implicated in the pathogenesis of rheumatoid arthritis (RA). Posttranslational glycosylation of CII could alter intracellular antigen processing, leading to the development of autoimmune T cell responses. To address this po