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Imobilization of β-glucosidase using the cellulose-binding domain of Bacillus subtilis endo-β-1,4-glucanase

✍ Scribed by Dong Ho Ahn; Hoon Kim; Moo Young Pack


Book ID
110369467
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
720 KB
Volume
19
Category
Article
ISSN
0141-5492

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Cleavage of bacillus subtilis endo-β-1,4
✍ Dong Ho Ahn; Hoon Kim; M. Y. Pack 📂 Article 📅 1993 🏛 Springer Netherlands 🌐 English ⚖ 370 KB

A protease secreted by B. megaterium ATCC 14945 was purified by ammonium sulfate fractionation, Q-Sepharose, Sephadex G-75, and hydroxyapatite chromatography and its molecular weight was estimated to be 38 kD. The purified protease caused the cleavage of 52 kD B. subtilis endo+1,4-glucanase expresse