Immunosuppressive effect of paracoccidioidomycosis sera on the proliferative response of normal mononuclear cells

✍ Scribed by Dumith Chequer-Bou-Habib; Maria Fátima Ferreira-da-Cruz; Bernardo Galvão-Castro

• Publisher: Springer Netherlands
• Year: 1992
• Tongue: English
• Weight: 618 KB
• Volume: 119
• Category: Article
• ISSN: 0301-486X
• DOI: 10.1007/bf00443935

✦ Synopsis

In this paper we relate that sera from paracoccidioidomycosis patients inhibited the mitogen-induced proliferative responses of normal mononuclear cells. Treatment of these sera with 2.5% polyethyleneglycol (PEG), a method classically used to precipitate immune complexes, significantly reduced their inhibitory activity. Immunoblot analysis of the PEG precipitates identified a 34-kDa polypeptide, recognized by rabbit anti-P. brasiliensis IgG. Patient mononuclear cells showed partial restoration of their proliferative capacity after 24 h culture in medium alone, which suggests release of membrane-bound molecules in the culture medium. These findings indicate that circulating P. brasiliensis antigens, complexed or not with antibodies, may play a negative immunoregulatory effect in the mitogen-induced proliferative responses of paracoccidioidomycosis patients.