Immunostick Colorimetric ELISA Assay for the Identification of Smoked Salmon, Trout and Bream
✍ Scribed by Esther Carrera; Teresa García; Ana Céspedes; Isabel González; Bernabé Sanz; Pablo E Hernández; Rosario Martín
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 164 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
✦ Synopsis
A colorimetric ELISA using immunostick tubes has been developed for the rapid identiÐcation of smoked salmon (Salmo salar), trout (Oncorhynchus mykiss) and bream (Brama raii). The assay uses polyclonal antibodies raised in rabbits against muscle-soluble proteins of smoked salmon (anti-SSP), trout (anti-TSP) and bream (anti-BSP) and rendered species-speciÐc by blocking them with the heterologous species of smoked Ðsh. The blocked antibodies were used for the immunorecognition of the smoked Ðsh samples bound to the paddles of immunostick tubes and the immunocomplex detected with peroxidaseconjugated goat anti-rabbit immunoglobulins. The blue colour developed by conversion of the peroxidase substrate allows unequivocal identiÐcation of S salar, O mykiss and B raii.