𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Immunosensing with amperometric detection, using galactosidase as label and P-aminophenyl-β-D-galactopyranoside as substrate

✍ Scribed by Már Mássen; Zheng Liu; Tetsuya Haruyama; Eiry Kobatake; Yoshihito Ikariyama; Masuo Aizawa

Book ID
102985805
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
574 KB
Volume
304
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

✦ Synopsis

p-Aminophenyl-P-D-galactopyranoside (PAPG) was shown to be a suitable substrate for the amperometric detection of galactosidase activity at neutral pH. The application of this amplification system for immunoassay was demonstrated. The product of the enzyme reaction, p-aminophenol (PAP), was detected at 200 mV, vs. Ag/AgCl, by flow-injection analysis (FIA), with a 50 nM detection limit. PAPG was hydrolyzed more than 2.5 times faster than p-nitrophenyl-P-o-galactopyranoside, by the enzyme. Both PAP and PAPG were stable at pH 7. The galactosidase concentration could be measured down to a concentration of 100 fh4, and mouse IgG could be assayed by sandwich immunoassay down to 700 fhL PAPG was found to be a promising reagent for heterogeneous systems, like the one described, and for homogenous assays of biological fluids.

📜 SIMILAR VOLUMES

β-Galactosidase assay using capillary el
β-Galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-β-D-galactopyranoside as substrate
✍ Michael J. Eggertson; Douglas B. Craig 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 50 KB

b-Galactosidase was incubated for 60 min with the fluorogenic substrate resorufin-b-D-galactopyranoside, which is converted by the action of the enzyme into resorufin and galactose. A 160 pL aliquot of reaction mixture was analyzed by capillary electrophoresis utilizing laser-induced fluorescence de

Chemiluminescent enzyme immunoassay for
Chemiluminescent enzyme immunoassay for alpha-fetoprotein using β-D-galactosidase as label and 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside as substrate
✍ Arakawa, H. ;Ikegami, T. ;Maeda, M. ;Tsuji, A. 📂 Article 📅 1993 🏛 John Wiley and Sons ⚖ 296 KB

## Abstract We developed a sensitive chemiluminescent sandwich‐type enzyme immunoassay (CLEIA) of alpha‐fetoprotein (AFP) using b̃‐D‐galactosidase (b̃‐gal) as a label and 5‐bromo‐4‐chloro‐3‐indolyl‐b̃‐D‐galactopyranoside as a substrate. The CL‐EIA for AFP was performed using two monoclonal antibodi