Immunoselection of oligodendrocytes by magnetic beads. I. Determination of antibody coupling parameters and cell binding conditions
✍ Scribed by D. H. Meier; C. Lagenaur; M. Schachner
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 902 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
Oligodendrocytes from early postnatal mouse cerebellum were isolated using polyacrylamide‐coated magnetic beads carrying monoclonal antibody to 04 cell surface antigen. Oligodendrocytes were enriched to a purity of 91 ± 4% starting from a mixed cell population containing approximately 1.5% antigen‐positive oligodendrocytes. Viability of 04 antigen‐positive oligodendrocytes was approximately 90% as judged by exclusion of trypan blue. Oligodendrocytes were recovered after detachment from the beads with a yield of 19 ± 6% and after collection by centrifugation onto glass coverslips with yields of approximately 6% of all 04 antigen‐positive cells. The final cell yield of oligodendrocytes is approximately 8 × 10^5^ cells/gram fresh cerebellar tissue.