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Immunoprecipitation of a male-specific polypeptide after in vitro translation of testicular poly(A)+RNA

✍ Scribed by Ulrich Lattermann; Ulrich Müller

Book ID: 104702952
Publisher: Springer
Year: 1988
Tongue: English
Weight: 377 KB
Volume: 79
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00282166

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✦ Synopsis

Poly(A)+ RNAs from male and female gonads of 20-day-old rats were translated in vitro using rabbit reticulocyte lysates. The resulting polypeptides were incubated with specific anti-male (anti-H-Y) antisera raised in female rats by intrasplenic immunization with syngeneic male skin. Using a second antibody, a male-specific polypeptide of molecular weight approximately 18,000 was immunoprecipitated. This male-specific polypeptide was not cotranslationally modified in vitro and appeared to be identical to serological H-Y antigen.

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