Immunology of the sperm-egg interaction
β Scribed by Stacey Lee Mandelbaum; Michael P. Diamond; Alan H. DeCherney
- Book ID
- 105085551
- Publisher
- Springer US
- Year
- 1986
- Tongue
- English
- Weight
- 529 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1573-7330
No coin nor oath required. For personal study only.
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## Abstract The interaction of sperm with the zonaβfree hamster egg was studied. Hamster sperm were capacitated in Tyrodes media, containing 50% heatβinactivated serum and used to inseminate zonaβfree eggs in BWW containing 10% serum. Capacitated sperm began fusing with eggs within the first hour o
## Abstract Immature oocytes were recovered from ovarian follicles at 0, 2, 4, 6, 8 and 10 hours after an ovulating injection of HCG. The envelopes (cumulus, corona cells and zona pellucida) of these oocytes were removed by enzymic treatment. The naked oocytes were incubated in vitro with capacitat