Immunological techniques: Dynamic imaging of the immune system

✍ Scribed by Peter Friedl

• Publisher: Elsevier Science
• Year: 2004
• Tongue: English
• Weight: 130 KB
• Volume: 16
• Category: Article
• ISSN: 0952-7915
• DOI: 10.1016/j.coi.2004.05.022

✦ Synopsis

of Wu ¨rzburg, where he serves as senior physician and clinical dermatologist. His research focus is the visualization of cellcell and cell-matrix interactions and dynamic cell patterning during immune cell interactions and tumor invasion by using 3D extracellular matrix-based cell culture models and advanced imaging procedures, such as time-resolved confocal reflection and fluorescence microscopy [1,2]. Recently, his laboratory moved into in vivo-imaging of tumor and immune cell migration by multi-photon microscopy [3], which complements the in vitro culture technology. These approaches have provided insight into the serial dynamics of T cells scanning across antigenpresenting cells (serial encounter model; [4]), the diversity of cell migration mechanisms (individual, collective; [5]), as well as novel escape responses in tumor cell migration (mesenchymalamoeboid transition, collectiveamoeboid transition; [6]).

Abbreviations APC antigen-presenting cell CCD charge-coupled device FRET fluorescence-resonance energy transfer GFP green fluorescent protein TCR T-cell receptor