Immunological relationships and a genetic interpretation of major and minor acidic proteins in human parotid saliva

✍ Scribed by Robert D. Friedman; Robert C. Karn

• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 669 KB
• Volume: 15
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00520197

✦ Synopsis

Isoelectric focusing was performed on parotid salivas selected for their electrophoretic phenotypes of proline-rich acidic salivary proteins. Fractions encompassing narrow pH regions were pooled and examined by polyacrylamide gel electrophoresis. Isoelectric focusing yielded partial purification of major and minor acidic proline-rich proteins which were subsequently compared by immunoelectrophoresis and double immunodiffusion against goat anti-human parotid saliva. Cross-reactivity without spurring between all fractions containing major Pr proteins in both immunoelectrophoresis and double immunodiffusion suggests that these proteins are immunologically very similar or identical.