Immunological quantitation of bacterial cells using a partition affinity ligand assay: A model study on the quantitation of streptococci B
✍ Scribed by T.G.I. Ling; M. Ramstorp; B. Mattiasson
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 517 KB
- Volume
- 122
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A new immunoassay for bacterial cells is described. Aqueous two-phase systems were used to separate the different reactants after binding, the principle being that in the direct assay free and bound reactants shall be recovered from different phases. In the competitive binding assay the bacteria are present in two forms-one native (to be quantified) and one modified (in fixed amount)--so that after binding and separation the complexes are recovered from different phases. '2SI-Labeled antibodies were used in the competitive assay and enzyme-labeled antibodies in the direct binding assay. In the system studied here streptococci were quantified. The direct enzyme immunoassay was more sensitive than the competitive radioimmunoassay. The smallest number of cells that could be quantified was 2500. Total time for one assay was 40-120 min.