Immunological Methods

✍ Scribed by Ivan Lefkovits, Benvenuto Pernis

• Publisher: Elsevier Inc, Academic Press
• Year: 1979
• Tongue: English
• Leaves: 460
• Edition: 1st
• Category: Library

✦ Table of Contents

Content:
Front Matter, Page iii
Copyright, Page iv
List of Contributors, Pages xv-xvi, Şefik Ş. Alkan, Domenico Bernoco, Daniel Ch. Brandt, Dietmar G. Braun, Humberto Cosenza, Bruce E. Elliott, C. Garrison Fathman, Luciana Forni, Ruedi Frech, Werner Haas, Kerstin Hild, Norman N. Iscove, Jean-Claude Jaton, Judith Johnson, Hansruedi Kiefer, Georges Köhler, Ivan Lefkovits, Alma L. Luzzati, Tommaso Meo, B.A. Moss, Markus Nabholz, et al.
Preface, Pages xvii-xix, Ivan Lefkovits, Benvenuto Pernis
Abbreviations List, Pages xxi-xxiii
1 - The Quality of Antibodies and Cellular Receptors, Pages 1-42, S. Fazekas de St. Groth
2 - The Isolation and Characterization of Immunoglobulins, Antibodies, and Their Constituent Polypeptide Chains, Pages 43-67, Jaton Jean-Claude, Daniel Ch. Brandt, Pierre Vassalli
3 - Peptide Mapping at the Nanomole Level, Pages 69-80, B.A. Kagarise
4 - Electrophoresis of Proteins in Polyacrylamide Slab Gels, Pages 81-105, Béla Takács
5 - Resolution of Immunoglobulin Patterns by Analytical Isoelectric Focusing, Pages 107-121, Dietmar G. Braun, Kerstin Hild, Andreas Ziegler
6 - Isolation of Monoclonal Antibody by Preparative Isoelectric Focusing in Horizontal Layers of Sephadex G-75, Pages 123-130, Wolfgang Schalch, Dietmar G. Braun
7 - Isotachophoresis of Immunoglobulins, Pages 131-136, Andreas Ziegler, Georges Köhler
8 - The Chemical Modification of Proteins, Haptens, and Solid Supports, Pages 137-150, Hansruedi Kiefer
9 - Reagents for Immunofluorescence and Their Use for Studying Lymphoid Cell Products, Pages 151-167, Luciana Forni
10 - Radiolabeling and Immunoprecipitation of Cell-Surface Macromolecules, Pages 169-179, J. Richard, L. Pink, Andreas Ziegler
11 - Haptenation of Viable Biological Carriers, Pages 181-195, Helmut M. Pohlit, Werner Haas, Harald von Boehmer
12 - Production and Assay of Murine Anti-Allotype Antisera, Pages 197-206, Judith Johnson
13 - Preparation of Mouse Antisera against Histocompatibility Antigens, Pages 207-216, C. Garrison Fathman
14 - Technique of HLA Typing by Complement-Dependent Lympholysis, Pages 217-226, John W. Stocker, Domenico Bernoco
15 - The MLR Test in the Mouse, Pages 227-239, Tommaso Meo
16 - A Sensitive Method for the Separation of Rosette-Forming Cells, Pages 241-259, Bruce E. Elliott
17 - The Use of Protein A Rosettes to Detect Cell-Surface Antigens, Pages 261-267, Judith Johnson
18 - Hapten–Gelatin Gels Used as Adsorbents for Separation of Hapten-Specific B Lymphocytes, Pages 269-276, Werner Haas
19 - Assay for Plaque-Forming Cells, Pages 277-285, Ivan Lefkovits, Humberto Cosenza
20 - Plaquing and Recovery of Individual Antibody-Producing Cells, Pages 287-290, Marc Shulman
21 - Assay for Specific Alloantigen-Binding T Cells Activated in the Mixed Lymphocyte Reaction, Pages 291-308, Bruce E. Elliott, Zoltán Nagy, Markus Nabholz
22 - Assay for Antigen-Specific T-Cell Proliferation in Mice, Pages 309-315, Şefik Ş. Alkan
23 - Antigen-Specific Helper T-Cell Factor and Its Acceptor, Pages 317-325, Michael J. Taussig
24 - In Vitro Immunization of Dissociated Murine Spleen Cells, Pages 327-334, Max H. Schreier
25 - Induction of a Secondary Antibody Response in Vitro with Rabbit Peripheral Blood Lymphocytes, Pages 335-343, Alma L. Luzzati
26 - Induction of Immune Responses with Clonal Dominance at High Antibody Levels, Pages 345-354, Dietmar G. Braun
27 - Limiting Dilution Analysis, Pages 355-370, Ivan Lefkovits
28 - Establishment and Maintenance of Murine Lymphoid Cell Lines in Culture, Pages 371-378, Max H. Schreier, Bernd J. Weimann
29 - Clonal Growth of Cells in Semisolid or Viscous Medium, Pages 379-385, Norman N. Iscove, Max H. Schreier
30 - Preparation of Sendai Virus for Cell Fusion, Pages 387-390, Reet Tees
31 - Fusion of Lymphocytes, Pages 391-395, Georges Köhler
32 - Soft Agar Cloning of Lymphoid Tumor Lines: Detection of Hybrid Clones with Anti-SRBC Activity, Pages 397-401, Georges Kohler
33 - Isotope Laboratory, Pages 403-417, Helmut M. Pohlit, Jürg Widmer, Ruedi Frech
34 - Analysis of Immunological Data, Pages 419-456, Alberto Piazza
Subject Index, Pages 457-467