Immunological memory function of the T and B cell types: distribution over mouse spleen and lymph nodes
✍ Scribed by T. J. Romano; J. J. Mond; G. J. Thorbecke
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 497 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Abstract
Spleens from LAF~1~ mice injected intravenously with sheep erythrocytes (SE) are relatively rich in memory T cells early in the immune response (1 to 3 days) and rich in memory B cells as the response progresses (2 weeks or more). Marked cooperation for the secondary immune response in vitro was obtained by combining 10^6^ spleen cells from LAF~1~ mice, taken 2 days after intravenous priming with SE, with 10^7^ spleen cells from day 14 primed mice. The results indicate relative deficiencies in the spleen for B memory cells on days 1 to 2 and for T memory cells on day 14 after priming. Day – 14, but not day – 2, immune lymph node (LN) cells could replace the day – 2 spleen cells (anti‐Thy 1.2 sensitive) in the in vitro cooperation with day – 14 immune spleen cells.
Immune spleen cells taken 4 to 7 days after priming contain more equivalent numbers of B and T memory cells, but 10 to 7 days after transfer of such immune spleen cells without SE into irradiated recipients the T memory cells were again more prominent in lymph node and the B memory cells in spleen as shown by in vitro cooperation studies. These results suggest that during the second week after intravenous injection of SE relatively more T than B memory cells migrate from spleen to lymph node, resulting in an imbalance in the splenic memory cell population favoring B memory cell function.