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Immunological determination and size characterization of poly(ADP-ribose) synthesized in vitro and in vivo

โœ Scribed by El Bachir Affar; Patrick J. Duriez; Rashmi G. Shah; Eric Winstall; Marc Germain; Claudia Boucher; Sylvie Bourassa; James B. Kirkland; Guy G. Poirier


Book ID
117481859
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
348 KB
Volume
1428
Category
Article
ISSN
0304-4165

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โœ Hector Juarez-Salinas; Hilda Mendoza-Alvarez; Viktorya Levi; Myron K. Jacobson; ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 793 KB

Methodology for the routine and simultaneous determination of the linear and branched residues of poly(ADP-ribose) is described. The main features of the procedure consist of the isolation of poly(ADP-ribose) by affinity chromatography; enzymatic digestion of the polymer to the unique nucleosides ri