Immunological analysis of apolipophorin-III in the haemolymph, ovaries, and testes of the fall webworm, Hyphantria cunea (Drury)

✍ Scribed by Hwa Kyung Yun; Hak R. Kim

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 733 KB
• Volume: 31
• Category: Article
• ISSN: 0739-4462
• DOI: 10.1002/(sici)1520-6327(1996)31:4<413::aid-arch4>3.0.co;2-w

✦ Synopsis

Apolipophorin-Ill (apolp-ill) was purified from the haemolymph of adult Hyphantrid tuned (Drury) by KBr density gradient ultracentrifugation, gel filtration (Sephadex G-100) and ion exchange chromatography (CM-52), and its characteristics, molecular weight, tissue distribution, and sites of synthesis were examined. Molecular weight of apolp-Ill was estimated to be 18 kDa. By electrophoretic analysis o n gels of male and female haemolyrnph from diverse developmental stages, apolp-Ill was shown t o be present in all stages.

Western blotting was carried out to show that purified free apolp-Ill is identical t o apolp-Ill associated w i t h adult lipophorin. lrnrnunological analysis also

showed that apolp-Ill is present in the ovary and the testis and in the case of testis, apolp-Ill is heavily accumulated in the cyst. ApoLp-Ill is synthesized in larval and adult Cat body but not in adult testis. Autoradiography following incubation of ['4C]apoLp-lII with testis showed that apolp-Ill was taken u p into testis.