Immunologic Methods in Steroid Determination

✍ Scribed by David Pressman, Allan L. Grossberg (auth.), Fernand G. Péron, Burton V. Caldwell (eds.)

• Publisher: Springer US
• Year: 1970
• Tongue: English
• Leaves: 240
• Edition: 1
• Category: Library

✦ Synopsis

The two main goals of the symposium upon which this volume is based were 1) to cement together knowledge presently available in the field of antibodies to steroids and obtainable only under separate covers in different journals and books, and 2) to present new data which could lead to a more complete understanding of physiologic phenomena like those occurring during the menstrual cycle, or to the elucidation of the mechanisms involved in steroid-protection interaction, or to the practical application of immunologic techniques to measurements of steroid hor­ mones. These techniques are extremely sensitive and can measure levels of steroid on the same order of magnitude as the radioisotope methods. However, the latter are much more laborious and costly which limits their use in many cases to the research laboratory. But the immunologic techniques generally classified as radioimmunoassay, are fraught with difficulties and problems which must be overcome. Fortunately, perhaps, the subject of immunologic techniques as applied to steroid determination is the child of radioimmunoassay of proteins, so to speak. Many of the problems which confront the former have been resolved in the latter instance. Thus, we are in an advantageous position because we are aware of the biologic and technical problems of the earlier radioimmunoassay techniques. Similar experiences have been reported in the book about the use of immunologic techniques for determination of steroid hormones.

✦ Table of Contents

Front Matter....Pages i-xii
Front Matter....Pages xv-xv
Structural Basis of Antibody Specificity....Pages 1-14
Early Approaches to Production, Analysis, and Use of Steroid-Specific Antisera....Pages 15-39
Specificities of Steroid Antibodies....Pages 41-62
Front Matter....Pages N1-N1
Preparation and Purification of Antibodies to Steroids....Pages 63-86
Solid-Phase Radioimmunoassay of Serum Estradiol-17β: A Semi-Automated Approach....Pages 87-112
Radioimmunoassay of Plasma Estrogens: Use of Polymerized Antibodies....Pages 113-126
Solid-Phase Radioimmunoassay of Steroids....Pages 127-147
Hapten-Radioimmunoassay for Steroid Hormones....Pages 149-173
The Use of Affinity Chromatography in the Purification of Steroid-Binding Proteins....Pages 175-182
Front Matter....Pages N3-N3
Physiologic Studies Using Antibodies to Steroids....Pages 183-197
The Use of Antibodies as a Tool in Studies in Reproductive Physiology....Pages 199-223
Summary....Pages 225-229
Back Matter....Pages 231-232

✦ Subjects

Immunology