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Immunolocalization of proton-ATPase in the gills of the elasmobranch,Squalus acanthias

✍ Scribed by Wilson, Jonathan M.; Randall, David J.; Vogl, A. Wayne; Iwama, George K.

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 307 KB
Volume: 278
Category: Article
ISSN: 0022-104X
DOI: 10.1002/(sici)1097-010x(19970601)278:2<78::aid-jez2>3.0.co;2-1

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✦ Synopsis

Proton-ATPase was localized to mitochondria-rich cells in the interlamellar region of the gills of the elasmobranch, Squalus acanthias. Localization was accomplished using a polyclonal antibody specific for the 70 kDa subunit of the (V-type) proton-ATPase as confirmed by Western blot analysis. In addition, significant levels of N-ethymaleimide sensitive ATPase activity (0.116 +/- 0.026 mumol Pi.mg-1 protein.h-1) were also measured in crude gill membrane preparations. These data provide, for the first time, direct evidence of the localization of elements possibly involved in branchial acid-base (or ionic) regulation in elasmobranchs.

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