Immunolocalization of mitochondrial 3-hydroxy-3-methylgutaryl CoA synthase in rat liver

✍ Scribed by Teresa Royo; Maria J. Pedragosa; José Ayté; Gabriel Gil-Gómez; Senén Vilaró; Fausto G. Hegardt

• Book ID: 102886450
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 902 KB
• Volume: 162
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041620112

✦ Synopsis

We report the preparation of specific polyclonal antibodies raised against two synthetic peptides deduced from the cDNA sequence for the rat liver mitochondrial 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) synthase gene. Immunoelectron microscopy using these antibodies on hepatic cryoultrathin sections confirms the mitochondrial localization of this protein in hepatocytes. Immunofluorescence microscopy on frozen sections of adult rat liver revealed fluorescence inside all hepatocytes, with no evidence of zonation, indicating that ketogenesis may not be limited to specific regions of rat liver but i s extended to all hepatocytes. o 1995 ~i l e y -~i s s ,