Immunohistochemical study of melanocytic nevus and malignant melanoma with monoclonal antibodies against s-100 subunits
✍ Scribed by Kwang Hyun Cho; Ken Hashimoto; Yoshiki Taniguchi; Teresa Pietruk; Richard J. Zarbo; Teisa An
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 793 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0008-543X
No coin nor oath required. For personal study only.
✦ Synopsis
Immunohistochemical localization of S-100 protein a and B subunits in the cells of melanocytic nevi and malignant melanomas was studied by using monoclonal antibodies directed against each subunit. Although polyclonal anti-S-100 reactivities have been demonstrated uniformly in all nevus cells and melanoma cells, monoclonal anti-S-100a and anti-S-10oB reactivities were either absent or rarely found in ordinary junctional nevi or junctional nests of ordinary compound nevi. However, in the junctional nests of dysplastic junctional nevi and junctional components of dysplastic compound nevi, monoclonal anti-S-100a reactivity become more frequent, whereas monoclonal anti-S-1008 reactivity remains negative. In the superficial variety of melanomas such as superficial spreading melanoma and lentigo maligna melanoma, monoclonal anti-S-100B is nonreactive until vertical growth or invasiveness begins. Most nodular melanomas are positively stained with both monoclonal anti-S-100a and anti-S-100@. It is suggested that monoclonal anti-S-100a can be an indicator of active junctional nevus of melanocytic nevi and the reactivity with monoclonal anti-S-100B may be related to vertical progression of superficial spreading melanomas and lentigo maligna melanomas. Cancer 66:765-771,1990.
-100 PROTEIN is an acidic calcium-binding protein S distributed abundantly in the nervous system of the wide variety of the vertebrates.' S-100 protein was originally regarded as nervous system-specific protein. However, it is now known to be present in several nonneural cells, such as Langerhans' cells, melanocytes, and chondrocytes, and in tumors derived from these cell In the study of melanocytic nevus and malignant melanoma, staining with polyclonal antibody prepared against S-100 protein has proven to be a valuable tool.' Isobe et aL.*-" have demonstrated that S-100 protein isolated from the brain is not a single protein, but a mixture of at least three similar proteins, S-100 ao, S-100 a, and S-100 b, with a subunit composition of a01 (S-100 ao), aP (S-100 a), and P/3 (S-100 b). They also showed that brain S-100 is predominantly composed ofthe P subunit containing protein, s-100 b (PP) and 5-100 a (a@).
By using specific monoclonal antibodies directed against either the a or the P subunit, we have demonstrated that these subunits could be used in the grading of invasiveness of borderline melanomas.