Diethylstilbestrol (DES) treatment of duced enough renin to raise the level of circulating a male Syrian hamster resulted in the development of a renin, plasma renin activity (PRA) was determined for renal tumor and its widely scattered serosal metastases.
The Cells in both the primary tumor and metastatic nodules PRA Of the host hamsters was 'Ompared to the contained secretory granules. The tumors were transplanted serially into DES-supported and non-DES-sup-
MATERIALS AND METHODS
ported host hamsters until DES-independent tumors
The Syrian hamsters used in this study were obtained developed. Rabbit antiserum to mouse salivary renin from the Dodge hamstel-colony. The adult male and and rabbit antiserum to rat kidney resin were reacted female hamsters used in this study were either Golden with sections of the primary tumor, metastatic nodules, or Kirkman albino hamsters. The Kirkman albino hamand all transport tumors. The sections were stained by ster (Dodge, 1984) has a white coat, pink ears, and light the p ~p and Vector-mC-Ap procedures. Renin-posipink to dark pink eyes. Data from breeding studies tive material was in all tumors. plasma renin suggest that this hamster be classified as a "spurious" activity (pRA) was determined for the host hamsters albino. The hamsters used in this study were over 2 months of age and had a n average weight of 100 gm.
the PRA values that had been determined for normal tic cages (12rr 14u, 12") with stainless-steel wire non-DES-treated male and female hamsters. It was tops. Corncob bedding and pine-shavings bedding were found that the average PRA values of host hamsters used and changed once or twice a week as needed. The carrying the tumor transplants were significantly hamster room was maintained between 65-70 O F , and higher than the normal PRA values.
there was no extraneous noise. The ambient lighting conditions were 15 hr of light from 1700 to 0800 hr and 9 hr of dark from 0800 to 1700 hr. The reverse darklight cycle was necessary to permit breeding during the workday. The diet consisted of rodent and breeding lab 0 1988 ALAN R. LISS, INC. host hamsters carrying the tumor baseline PRA of adult male and female hamsters.
the tumor and 'Ompared to Individual hamsters were maintained in metal or plas-