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Immunohistochemical localization of β1-integrins in anterior cruciate and medial collateral ligaments of human and rabbit

✍ Scribed by Dirk S. Gesink; Hector O. Pacheco; Scott D. Kuiper; Paul J. Schreck; David Amiel; Dr. Wayne H. Akeson; Virgil L. Woods Jr.

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
375 KB
Volume
10
Category
Article
ISSN
0736-0266

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✦ Synopsis

Abstract

The integrins are a family of adhesion‐mediating cell surface receptors that play critical roles in cell‐extracellular matrix interactions and have been shown to be important in the healing response in several tissues. We have studied integrin expression in normal human and rabbit anterior cruciate (ACL) and medial collateral (MCL) ligaments of the knee as a preamble to studies of β~1~‐integrin expression in healing ligaments. Histologic sections of human and rabbit ACL and MCL were probed for integrin expression utilizing integrin‐specific monoclonal antibodies (mAbs) followed by immunoperoxidase detection. Staining of human specimens with mAbs revealed the presence of β~1~‐, α~1~‐, and α~5~‐integrin chains on the tissue fibroblasts of both ACL and MCL, while staining of rabbit specimens with rabbit integrin‐reactive monoclonals revealed the presence of β~1~‐ and α~5~‐integrin on these ligaments. Equivalent amounts of the integrins studied were present on normal ACL and MCL. We conclude that the rabbit is an appropriate model for analyzing the expression and functional role of integrins in ligament wound healing.

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