✦ LIBER ✦

Immunohistochemical detection of P-glycoprotein in teleost tissues using mammalian polyclonal and monoclonal antibodies

✍ Scribed by Hemmer, Michael J. ;Courtney, Lee A. ;Ortego, Lisa S.

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 991 KB
Volume: 272
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402720109

No coin nor oath required. For personal study only.

✦ Synopsis

Mammalian P-glycoprotein is a highly conserved 170-kD integral plasma membrane protein functioning as an energy-dependent efflux pump of exogenous and endogenous lipophilic aromatic compounds entering the cell by diffusion. In this study, the tissue specificities of one polyclonal (pAb) and three monoclonal (mAbs) antibodies t o mammalian P-glycoprotein were identified in paraffin-embedded, parasagittal whole-body sections of the guppy Poecilia reticulata. Polyclonal antibody mdr(Ab-1) and mAbs C219, C494, and JSB-1 demonstrated differential staining patterns in the following tissues: bile canaliculi in the liver, exocrine pancreas, lumenal surface of the intestinal epithelium, renal tubules, interrenal tissue, branchial blood vessels, gas gland, pseudobranch, and the gill transverse septa. Positive P-glycoprotein expression in P reticulata

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